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rabbit anti ac p53  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti ac p53
    Rabbit Anti Ac P53, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac+p53+rabbit/pm40774472-66-0-9
    Average 86 stars, based on 1 article reviews
    rabbit anti ac p53 - by Bioz Stars, 2026-11
    86/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Sirtuin-2 Is Involved in the Regulation of Claudin-4 Expression and Paracellular Barrier Function in Keratinocytes.
    Article Snippet: Claudin‐1 (CLDN1) and CLDN4 are highly expressed in keratinocytes and may function as a paracellular barrier to water and small molecules.. The physiological function of CLDN4 has not been fully understood, whereas dysfunction of CLDN1 is involved in the pathophysiology of allergy and inflammatory diseases.. Here, we found that the protein level of CLDN4 in the skin tissues of 36‐week‐old mice was lower than that in 6‐week‐old mice.



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    Idebenone Plays a Protective Role by Reducing the Expression of Apoptotic Protein P53/Caspase3. ( A )( E ) The expression of P53Ac protein was detected by western blot. ( B )( F ) The expression of Caspase3 protein was detected by western blot. ( C ) The expression of Caspase3 protein was detected by real-time PCR. ( D ) The expression of P53 protein was detected by real-time PCR, * p < 0.05, ** p < 0.01 and *** p < 0.001

    Journal: Neurochemical Research

    Article Title: Idebenone Antagonizes P53-Mediated Neuronal Oxidative Stress Injury by Regulating CD38-SIRT3 Protein Level

    doi: 10.1007/s11064-024-04189-7

    Figure Lengend Snippet: Idebenone Plays a Protective Role by Reducing the Expression of Apoptotic Protein P53/Caspase3. ( A )( E ) The expression of P53Ac protein was detected by western blot. ( B )( F ) The expression of Caspase3 protein was detected by western blot. ( C ) The expression of Caspase3 protein was detected by real-time PCR. ( D ) The expression of P53 protein was detected by real-time PCR, * p < 0.05, ** p < 0.01 and *** p < 0.001

    Article Snippet: Rat anti-mouse P53 protein polyclonal antibody (Bioss Biotech Co., LTD, China), rabbit anti-mouse Caspase3 polyclonal antibody (Bioss Biotech Co., LTD, China), rabbit anti-mouse SIRT3 polyclonal antibody (Bioss Biotech Co., LTD, China), rabbit anti-mouse P53-AC monoclonal antibody (Abcam, England), and rabbit anti-mouse β-actin monoclonal antibody (Bioss Biotech Co., LTD, China) were separately incubated with protein-transferred membranes overnight at 4℃, and then they were washed with TBST for 3 times.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Idebenone Induces the Expression of SIRT3 in SIRT3-Down-Regulated HT22 Cells and the Deacetylation of P53Ac. ( A ) The expression of P53Ac protein was detected by western blot after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( B ) The expression of SIRT3 protein was detected by western blot after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( C ) The expression of SIRT3 was detected by real-time PCR after SIRT3 down-regulated. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( D ) The expression of P53 protein was detected by real-time PCR after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( E ) The expression of SIRT3 protein was detected by real-time PCR after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001

    Journal: Neurochemical Research

    Article Title: Idebenone Antagonizes P53-Mediated Neuronal Oxidative Stress Injury by Regulating CD38-SIRT3 Protein Level

    doi: 10.1007/s11064-024-04189-7

    Figure Lengend Snippet: Idebenone Induces the Expression of SIRT3 in SIRT3-Down-Regulated HT22 Cells and the Deacetylation of P53Ac. ( A ) The expression of P53Ac protein was detected by western blot after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( B ) The expression of SIRT3 protein was detected by western blot after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( C ) The expression of SIRT3 was detected by real-time PCR after SIRT3 down-regulated. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( D ) The expression of P53 protein was detected by real-time PCR after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 ( E ) The expression of SIRT3 protein was detected by real-time PCR after SIRT3 down-regulated in various groups. * p < 0.05, ** p < 0.01 and *** p < 0.001

    Article Snippet: Rat anti-mouse P53 protein polyclonal antibody (Bioss Biotech Co., LTD, China), rabbit anti-mouse Caspase3 polyclonal antibody (Bioss Biotech Co., LTD, China), rabbit anti-mouse SIRT3 polyclonal antibody (Bioss Biotech Co., LTD, China), rabbit anti-mouse P53-AC monoclonal antibody (Abcam, England), and rabbit anti-mouse β-actin monoclonal antibody (Bioss Biotech Co., LTD, China) were separately incubated with protein-transferred membranes overnight at 4℃, and then they were washed with TBST for 3 times.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction